ABSTRACT
Objective:To investigate the correlation between the expression of vascular endothelial growth factor (VEGF), MMP-2, MMP-9 and the clinicopathology and prognosis of patients with hepatocellular carcinoma (HCC), and to provide evidence for the occurrence and development of HCC.Methods:From April 2015 to March 2016, 95 HCC tissues were resected in Yiwu Central Hospital.Forty-fivenormal liver tissues which were resected due to liver trauma were selected as control group.Immunohistochemical staining was used to detect the expression of VEGF, MMP-2 and MMP-9 in HCC and normal liver tissues, and the correlation between the expression of VEGF, MMP-2, MMP-9 and clinical pathology and prognosis was analyzed.Results:The positive expression rates of VEGF, MMP-2 and MMP-9 in the HCC tissues were significantly higher than those in the normal liver tissues (81.05%, 75.79%, 84.21% vs.42.22%, 33.33%, 48.89%)(χ 2=21.364, 23.397, 19.265, all P<0.05). The positive expression rates of VEGF, MMP-2 and MMP-9 in HCC were significantly increased in patients with poor differentiation, TNM stage Ⅲ-Ⅳ and vascular invasion (all P<0.05). The survival of patients with negative expression of VEGF, MMP-2 and MMP-9 in HCC tissues was significantly better than that of patients with positive expression (log χ 2=3.271, 2.975, 3.065, all P<0.05). Conclusion:The high expression of VEGF, MMP-2 and MMP-9 plays an important role in the growth, invasion and metastasis of HCC tissues.Therefore, VEGF, MMP-2 and MMP-9 can be used as biological indicators of the progression of HCC.
ABSTRACT
Objective@#To investigate the level and clinical significance of miRNA-106a in non-small cell lung cancer tissues.@*Methods@#Paraffin-embedded specimens of non-small cell lung cancer tissues and adjacent tissues from 80 patients with non-small cell lung cancer from January 2012 to December 2013 in Yiwu Central Hospital were selected in this study.The real-time fluorescence quantitative polymerase chain reaction (QRT-PCR) was used to determine the level of miRNA-106a in lung cancer tissues and adjacent tissues.@*Results@#The level of miRNA-106a in non-small cell lung cancer tissues (2.42±0.23) was higher than that in adjacent tissues (1.00±0.06) (t=53.433, P=0.000). The miRNA-106a levels in lung cancer tissues of stage Ⅲ-Ⅳ, lymph node metastasis and recurrence time<6 months were high than those of the stage Ⅰ-Ⅱ, no lymph node metastasis and recurrence time ≥ 6 months(t=7.641, 11.115, 2.183, P=0.000, 0.000, 0.032). The level of miRNA-106a was not associated with age, gender, pathological type, degree of differentiation and vascular invasion (all P>0.05). The level of miRNA-106a in non-small cell lung cancer tissues was cut by 2.12.The area under the ROC curve for the diagnosis of non-small cell lung cancer was 0.823(95% CI=0.820-0.825, P=0.000), and the sensitivity was 54.37%, the specificity was 89.21%.The cumulative survival rate and progression-free survival rate of patients with low-expression of miRNA-106a in non-small cell lung cancer were higher than those with high expression of miRNA-106a (P=0.027, 0.012).@*Conclusion@#The miRNA-106a level is elevated in non-small cell lung cancer tissues.The miRNA-106a may be involved in the development of non-small cell lung cancer and is of great value in the diagnosis and prognosis evaluation of non-small cell lung cancer.
ABSTRACT
Objective To investigate the level and clinical significance of miRNA －106a in non－small cell lung cancer tissues.Methods Paraffin－embedded specimens of non －small cell lung cancer tissues and adjacent tissues from 80 patients with non －small cell lung cancer from January 2012 to December 2013 in Yiwu Central Hospital were selected in this study.The real－time fluorescence quantitative polymerase chain reaction (QRT－PCR) was used to determine the level of miRNA －106a in lung cancer tissues and adjacent tissues.Results The level of miRNA－106a in non－small cell lung cancer tissues (2.42 ±0.23) was higher than that in adjacent tissues (1.00 ± 0.06) (t＝53.433,P＝0.000).The miRNA －106a levels in lung cancer tissues of stage Ⅲ －Ⅳ,lymph node metastasis and recurrence time ＜6 months were high than those of the stage Ⅰ－Ⅱ,no lymph node metastasis and recurrence time≥6 months(t＝7.641,11.115,2.183,P＝0.000,0.000,0.032).The level of miRNA－106a was not associated with age ,gender,pathological type,degree of differentiation and vascular invasion (all P＞0.05).The level of miRNA－106a in non－small cell lung cancer tissues was cut by 2.12.The area under the ROC curve for the diagnosis of non －small cell lung cancer was 0.823(95%CI＝0.820－0.825,P＝0.000),and the sensitivity was 54.37%,the specificity was 89.21%.The cumulative survival rate and progression －free survival rate of patients with low－expression of miRNA －106a in non －small cell lung cancer were higher than those with high expression of miRNA－106a (P＝0.027,0.012).Conclusion The miRNA －106a level is elevated in non －small cell lung cancer tissues.The miRNA－106a may be involved in the development of non －small cell lung cancer and is of great value in the diagnosis and prognosis evaluation of non －small cell lung cancer.